Plasmid Preparation:Article Title: Recognition of a translocation motif in the regulator HpaA from Xanthomonas euvesicatoria is controlled by the type III secretion chaperone HpaB
Article Snippet: Destination vectors pAGM9121 pUC19-derived vector, lacZα fragment flanked by BpiI sites; SmR (Addgene #51833; Weber et al., 2011) pAGM1311 pUC19-derived level -1 vector, lacZα fragment flanked by BsaI sites; KmR (Addgene #47983; Weber et al., 2011) pICH41021 Derivative of pUC19 with mutated BsaI site; ApR Gift from S. Marillonnet pICH41276 pUC19-derived level 0 vector, lacZα fragment flanked by BpiI sites; external BsaI sites generate GCTT / CGCT overhangs; SmR (Addgene #47994; Weber et al., 2011) pICH41295 pUC19-derived level 0 vector, lacZα fragment flanked by BpiI sites; external BsaI sites generate GGAG / AATG overhangs; SmR (Addgene #47997; Weber et al., 2011) pICH41308 pUC19-derived level 0 vector, lacZα fragment flanked by BpiI sites; external BsaI sites generate AATG / GCTT overhangs; SmR (Addgene #47998; Weber et al., 2011) pICH41331 pUC19-derived level 0 vector, lacZα fragment flanked by BpiI sites; external BsaI sites generate GGAG / CGCT overhangs; SmR (Addgene #47999; Weber et al., 2011) pICH47742 Level 1 destination vector derived from pBIN19 and pUC19, lacZα fragment flanked by BsaI sites, for level M position 2'; ApR (Addgene #48001; Weber et al., 2011) pICH47751 Level 1 destination vector derived from pBIN19 and pUC19, lacZα fragment flanked by BsaI sites, for level M position 3; ApR (Addgene #48002; Weber et al., 2011) pICH47781 Level 1 destination vector derived from pBIN19 and pUC19, lacZα fragment flanked by BsaI sites, for level M position 6; ApR (Addgene #48005; Weber et al., 2011) pICH47811 Level 1 destination vector derived from pBIN19 and pUC19, lacZα fragment flanked by BsaI sites, for level M position 2; ApR (Addgene #48008; Weber et al., 2011) pAGM8031 Level M vector derived from pBIN19 and pUC19, lacZα fragment flanked by BpiI sites; SmR (Addgene #48037; Weber et al., 2011) pAGM8079 Level M vector derived from pBIN19 and pUC19, lacZα fragment flanked by BpiI sites; SmR (Addgene #48041; Weber et al., 2011) pICH75322 Level P vector derived from pPZP200 and pUC19, lacZα fragment flanked by BsaI sites, ColE1 and pVS1 ori, KmR (Addgene #48051; Weber et al., 2011)
Derivative Assay:Article Title: Recognition of a translocation motif in the regulator HpaA from Xanthomonas euvesicatoria is controlled by the type III secretion chaperone HpaB
Article Snippet: Destination vectors pAGM9121 pUC19-derived vector, lacZα fragment flanked by BpiI sites; SmR (Addgene #51833; Weber et al., 2011) pAGM1311 pUC19-derived level -1 vector, lacZα fragment flanked by BsaI sites; KmR (Addgene #47983; Weber et al., 2011) pICH41021 Derivative of pUC19 with mutated BsaI site; ApR Gift from S. Marillonnet pICH41276 pUC19-derived level 0 vector, lacZα fragment flanked by BpiI sites; external BsaI sites generate GCTT / CGCT overhangs; SmR (Addgene #47994; Weber et al., 2011) pICH41295 pUC19-derived level 0 vector, lacZα fragment flanked by BpiI sites; external BsaI sites generate GGAG / AATG overhangs; SmR (Addgene #47997; Weber et al., 2011) pICH41308 pUC19-derived level 0 vector, lacZα fragment flanked by BpiI sites; external BsaI sites generate AATG / GCTT overhangs; SmR (Addgene #47998; Weber et al., 2011) pICH41331 pUC19-derived level 0 vector, lacZα fragment flanked by BpiI sites; external BsaI sites generate GGAG / CGCT overhangs; SmR (Addgene #47999; Weber et al., 2011) pICH47742 Level 1 destination vector derived from pBIN19 and pUC19, lacZα fragment flanked by BsaI sites, for level M position 2'; ApR (Addgene #48001; Weber et al., 2011) pICH47751 Level 1 destination vector derived from pBIN19 and pUC19, lacZα fragment flanked by BsaI sites, for level M position 3; ApR (Addgene #48002; Weber et al., 2011) pICH47781 Level 1 destination vector derived from pBIN19 and pUC19, lacZα fragment flanked by BsaI sites, for level M position 6; ApR (Addgene #48005; Weber et al., 2011) pICH47811 Level 1 destination vector derived from pBIN19 and pUC19, lacZα fragment flanked by BsaI sites, for level M position 2; ApR (Addgene #48008; Weber et al., 2011) pAGM8031 Level M vector derived from pBIN19 and pUC19, lacZα fragment flanked by BpiI sites; SmR (Addgene #48037; Weber et al., 2011) pAGM8079 Level M vector derived from pBIN19 and pUC19, lacZα fragment flanked by BpiI sites; SmR (Addgene #48041; Weber et al., 2011) pICH75322 Level P vector derived from pPZP200 and pUC19, lacZα fragment flanked by BsaI sites, ColE1 and pVS1 ori, KmR (Addgene #48051; Weber et al., 2011)
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